Using Rapid Prototyping System to Produce Chitosan Composite 3D Scaffolds for Bone Tissue Repair
| Autor: | I-Hsin Liu, 劉易昕 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 Rapid prototyping (RP) systems produce porous scaffolds with interconnecting pores with evenly distributed cells. This is beneficial for nutritional and metabolic substance transportation to stimulate cell growth, differentiation, and production of extracellular matrix (ECM). Chitosan stimulates the differentiation of bone cells to promote bone growth and has application in temporary orthopedic filling materials, screws, and plates. Previous studies have neglected porous scaffolds made by RP systems with chitosan and composite materials. We have implemented RP technology to produce chitosan (C), chitosan-genipin cross-linked (CG), and chitosan-pectin cross-linked (CP) porous scaffolds. Further, we have compared them with lyophilization produced chitosan scaffolds (CFD) to identify the physical and biological compatibility characteristics differences to assess the suitability of the aforementioned materials to facilitate osseointegration. Physical tests showed that the CFD material had the largest pore diameter when compared to other materials, which means that the degradation of CFD is comparatively faster. The CG and CP materials had the greatest elasticity, flexibility, and strength. Biocompatibility characteristics were determined through the observation of the osteoblast growth formed through a scanning electronic microscope, and growth and differentiation were obtained through the quantities of DNA, alkaline phosphatase, and collagen. Furthermore, the degree of pyritization was measured using Von Kossa calcium staining and calcium quantity. Results show that C, CG, and CP had higher measurements in DNA, calcium, and collagen when compared to CFD. Results show that the biocompatibility of the materials made from RP techniques were better than by lyophilization and the mechanical strength of compound materials was better than pure chitosan. We have concluded that RP produced CG and CP porous scaffolds were optimal materials for the application in osseointegration. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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