In vitro and in vivo photothermal destruction of tumors by gold nanorods and laser irradiation
| Autor: | Bruce Yao-Wen Liu, 劉耀文 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 Gold nanorods (AuNRs) have tunable plasmon-resonant absorption in near-infrared (NIR) region and excellent photothermal transduction, making them attractive for the next generation of cancer therapy. In this study, AuNRs with a longitudinal absorption peak at 800 nm were prepared by one-pot synthesis method, and are further characterized by absorption spectra, electron microscopy, zeta potential and inductively coupled plasma mass spectroscopy. Both in vitro and in vivo approaches were performed to evaluate AuNRs’ therapeutic efficacy. According to dual color fluorescent staining imaging, cell destruction greatly depends on influence of energy and the amount of nanorods taken up. Laser-induced heating was applied to investigate the therapeutic effect of AuNRs, where the alteration in tumor size and vascular development was examined in the mouse ear. In the experiments, murine breast tumor cells were first introduced into the ears of Balb/c female mice. As the tumors grew to about 30 mm3 in size at the 10th day, AuNRs (~10 μg) were intratumorally injected into the ears directly, followed by employment of laser irradiation. The results of histological section and size measurements indicated that the photothermal effects of AuNRs are significantly destructive to the tumor development and may lead to final removal of the solid tumor. These results suggested that AuNRs have great potential in future clinical phototherapeutic applications. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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