Effect of Andrographis paniculata and Lonicera japonica on the remedy of wound
| Autor: | Chih-Chien Tseng, 曾至堅 |
|---|---|
| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 In previous reports indicate that some herbs can be used as wound healing drug. Astragali Radix and Rehmanniae Radix can treat diabetic foot ulcer and promote the wound healing, SBD.4 of Angelica sinensis and madecassoside of Centella asiatica can repair wound. Recently, Andrographis paniculata is used as a traditional medicine for the treatment of respiratory disease, inflammation, hypertension, infectious injury, and snake bites. Some efficacy has been reported including reduced influenza attack rate, promoted vasodilation of lowering blood pressure, anti-clotting, inhibited cancer cell growth and treated thrombosis. And Lonicera japonica has anti-inflammatory effect, anti-bacteria, and anti-virus. L. japonica also can inhibit tumor proliferation. The aim of this study was for investigating the benefit of A. paniculata and Lonicera japonica flower, leaf and stem crude extract in the treatment of wound. After HaCa T or CCD-966SK cells treated with crude extract of A. paniculata had higher proliferation than those of control and other treated groups (L. japonica flower, leaf and stem crude extract) at 24 hr. After 48 hr treatment, crude extract of Lonicera japonica stem was higher proliferation than those of A. paniculata. In addition Andrographolide also can promote HaCa T cell proliferation, whereas Andrographolide could inhibit cell proliferation of A375 (Human melanoma cell) in high concentration, and it also inhibited melanoma cell viability and decreased secretion of melanin. The results suggest that crude extracts of A. paniculata stimulate cell proliferation and subsequently help wound recovery in a short time of injury. In the MMPs assay, A. paniculata crude extract can promote secretion of MMP-9, and one report has indicated that MMP-9 can reduce scar formation. Therefore, using A. paniculata crude extract treated wound which has potential to reduce scar formation. Using the A. paniculata crude extract to treat mice burn, the result of healing was very well, and the rate of healing process was no significant difference as compared with commercially burn medicine (sulfadiazine silver, positive control) and the complete healing time as early as 4 days as compared with the positive control. The result implies that the crude extract of A. paniculata is effective for promoting wound healing. In addition, the Andrographolide is pure compound from A. paniculata, it had potential effect to treat the burn. And in tissue sections staining, the result showed that the structure of burn skin which treated by Andrographolide was similar to the normal skin structure after wound healing. This result is inferred that the Andrographolide may be the crucial composition of A. paniculata for wound healing. Taken together, A. paniculata, L. japonica, and excavatolide B can promote wound repair, especially Andrographolide has potential to decrease scar formation. These herbs and pure compounds can be potentially developed as wound treatment drugs. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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