Transcriptome profiles for selective killing of oral cancer cells by Physalis peruviana-derived compound AN37
| Autor: | Yung-Ting Chang, 張詠婷 |
|---|---|
| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 AN37 is a pure compound derived from Physalis peruviana. In our previous study, AN37 displayed the selective killing ability in oral cancer cell. We found that treatment with AN37 causes more DNA damage in oral cancer cell (Ca9-22) than normal cell (human normal gingiva fibroblast, HGF-1). Cell cycle analysis indicates that AN37 can promote Ca9-22 to undergo Sub-G1 arrest than HGF-1. However, the detailed mechanism insight into selective killing of AN37 against oral cancer is still unknown. In this thesis, the systems biology strategy based on microarray study, bioinformatics, and real-time PCR were applied to investigate the selective killing mechanism of AN37. Ca9-22 and HGF-1 cells were treated with IC50 dose of AN37 (3.14 ?慊/ml) for 2, 8 and 24 h for microarray experiments. Using bioinformatics data mining tool- MetaCore™, networks and candidates with differential expressions between oral cancer and normal cells were screened. I choose DNA damage pathway map: “ATM and ATR regulation of G1/S checkpoint” and validation by quantitative reverse transcriptase–polymerase chain reaction (qRT-PCR). Finally, we identified 18 genes by the certification (ATRIP, BLM, BRCA1, CCNA1, CCND1, CCNE1, CDC25A, CDK4, CDKN1A, CHEK1, CHEK2, CLSPN, MDM2, NBN, NFBD1, NFKB1, NFKBIE, and USP1) providing selective killing gene candidates which warrants further investigation in the oral cancer therapy. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|