The association of Guanine nucleotide binding protein β3 C825T polymorphism with colorectal cancer
| Autor: | Meng-Li, 陳孟莉 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 Colorectal cancer represents the third most common cancer worldwide, and high prevalence in Taiwan region. It has been proposed that risk factors for colorectal cancer include increased age, gender (men are more predisposed to the development of colorectal cancer), the presence of inflammatory bowel disease, certain hereditary conditions and a family history of colorectal cancer. There are many studies indicated that single nucleotide polymorphisms (SNPs) are associated with some cancers and disease. G proteins, important components of several transmembrane receptors, are involved in the regulation of intracellular signaling pathways. Recently, C825T polymosphism of GNB3, a G protein β3 subunit gene, has been identified to generate a splicing variant GNB3s protein. This splice variant is associated with various malignant diseases. In this study, we investigated the possible association of GNB3 gene polymorphism with colorectal cancer in Taiwan region. Using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), the allele frequency of the C825T polymorphism was investigated in 84 patients with colorectal cancer and 171 age matched control ( group IIA, with disease risks, n = 103; group IIB, without disease risks, n = 68). The frequency of the GNB3 825T allele in patients with colorectal cancer was significantly lower than in controls (33.33% vs 62.57%, OR 3.34, p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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