Formulation design and evaluation of quantum dot-loaded nanocarriers for integrating bioimaging and anticancer therapy
| Autor: | Yu-Jie Huang, 黃裕傑 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 In this report, we comprehensively studied the formulation design, bioimaging, and delivery of drug-entrapped nanocarriers with quantum dots (QDs), for integrating diagnosis and therapy. This study is to use the two nanocarriers: nanostructured lipid carriers (NLCs) and liposomes. Nanocarriers were characterized by size, zeta potential, morphology, molecular environment, differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). The application of the nanocarriers for imaging and drug delivery was evaluated by cytotoxicity, cell migration, cellular uptake, in vivo real-time tumor monitoring, anddrug accumulation in tumors. (Part I) Different QDs were selected to load with NLCs, which called QDNLC, including lipophilic QDs (L-QDs), carboxyl functioned QDs (C-QDs) and polyethylene glycol QDs (P-QDs). Cytotoxicity of the nanoparticles against melanoma cells was superior to that of free camptothecin. The real-time bioimaging demonstrated that C-QDNLCs could maintain the signaling in tumors for at least 24 h. (Part II) C-QDs was incorporated into different liposomes. Cationic quantum dot liposomes may be the best nanocarriers selected from this work because of their release, high cytotoxicity, and significant fluorescence labeling on cells and solid tumors. The drug-loaded nanocarriers with QDs evaluated in this study afford a new potential method for simultaneous therapeutic and diagnostic purposes. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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