A novel electrochemical method for the detection of Escherichia coli in liquid samples.
| Autor: | Shu-Fan Tang, 湯淑帆 |
|---|---|
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The typical coliform testing method, MPN, is time-consuming at 48 hours per sample. In addition, further analysis is required to differentiate fecal coliforms from E. coli in samples. A simple and rapid technique based on electrochemical principles is developed to analyze E. coli in liquid samples. The method includes a laboratory-built potentiostat connected with a pair of electrodes and a water sample with media containing lauryl sulfate. The electrodes were immersed in the liquid solution. When a water sample was placed into contact with the electrodes, the current change observed was used to determine the concentration of E. coli. If the current change exceeded a certain threshold, it indicated that the test sample contained E. coli. If the current change exerted to a low extent, or not at all, it was determined that the sample maybe contained other microorganisms (e.g., Enterobacter, Citrobacter, or Klebsiella). The sensor was able to acquire sufficient data within four hours at initial concentrations of E. coli higher than 105 CFU/ mL, making it suitable for use in real-time monitoring of bacterial growth. This system has potential application in the detection of E. coli concentrations in water of various sources. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|