The studies of a novel soluble calcium phosphate for teeth remineralization
| Autor: | Chen-Feng Ma, 馬晨峰 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The purpose of this study was to develop a novel soluble calcium compound based teeth remineralization patches. The calcium lactate phosphate (CLP) was designed and synthesized by wet chemical method. The calcium oxide added to the mixture with various molar ratios of lactic acid and phosphoric acid under stirring for different time periods. After 1 hr, 3 hrs, 6 hrs, 12 hrs and 24 hrs synthesizing, the CLP solution was first filtered and then freeze dried (liquid phase). The crystalline structure, Ca/P ratio, solubility, functional groups and thermogravimetric properties of harvested CLP were analyzed by x-ray diffractometer (XRD), x-ray fluorescence (XRF), moisture analyzer, Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA), respectively. The double-layer teeth remineralization patches include material layer for good mucoadhesion and CLP releasing, while the backing layer for anti-adhesion. The in vitro remineralization test was conducted by using human teeth (etched by 1 M acetic acid for 3 mins). The experiment groups were deionized water (control) and artificial saliva (positive control). Teeth remineralization patches containing 1% CLP, 3% CLP and 5% CLP were used as experimental groups. Knoop Hardness Number (KHN) for etched enamel before and after treatment by patches from microhardness assay was utilized to index teeth remineralization. The measured solubility of CLP was 8.0 g/100 ml H2O at 25°C, and it is significantly higher than the solubility of amorphous calcium phosphate (< 0.01 g/100 ml H2O at 25°C). The XRD diagram for CLP showed no obvious diffraction peaks indicating the amorphous nature of CLP compounds. The Ca/P mole ratio was about 4.5 according to the XRF measurement. After applying teeth remineralization patches with 3% and 5% CLP to etched enamel surface for 15 mins, the % hardness recovery showed 39.7 ± 39.1% and 56.5 ± 43.8% (p < 0.05 by paired t-test). It is significantly higher than artificial saliva (-12.2 ± 19.2%). In addition, the remineralization effect of CLP also could be using as a dentinal tubules sealer. The pilot study of using CLP paste on dentinal tubules plugging of was undertaken. The feasibility of CLP used for teeth remineralization was proven. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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