Calcium signal plays a role during zebrafish myogenesis
| Autor: | Jen-Cheng Wei, 魏任徵 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Calcium signaling plays important physiological roles on muscle physiology. For example, enhancement of cellular calcium concentration causes short-term spasm, then make muscle faintness or pain. However, the function of intracellular calcium signaling is unclear during early myogenesis. In this thesis, we used 2-APB(intracellular calcium blocker) and caffeine((intracellular calcium inducer) to regulate intracellular calcium concentration and to investigate the effect caused by calcium during early myogenesis. Our data showed that 2-APB treated zebrafish embryos displayed some malformed phenotypes, such as defective somite and myofribril misalignment. We also found that myod signals were up-regulated and expressed disorderly in the 2-APB-treated embryos. Knock down of myod expression can reverse 2-APB-induced muscle defect phenotypes. Results from real-time PCR showed that desma, fbxo32, and nfatc1 were up regulated but cpped1 was down regulated in 2-APB-treated embryos. On the basis of these observations, we proposed a model to elucidate how intracellular calcium delicate regulates early myogenesis |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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