Effects of Pinus morrisonicola Hayata on insulin resistance prevention and antioxidative properties in high fructose-fed rats

Autor: Lin-Chen Huang, 黃遴蓁
Druh dokumentu: 學位論文 ; thesis
Popis: 99
Recent evidence strongly suggests that oxidative stress due to redox imbalance is highly associated with metabolic syndrome. However, Pinus morrisonicola Hay. needle (PM) have been shown to exerts anti-oxidation, anti-hypertension, anti-inflammatory and anti-atherosclerotic effects. Therefore, this study was investigated the effects of PM water extract (PWE) and ethanol extract (PEE) on metabolic syndrome in the high-fructose-fed rat model. Adult male Sprague-Dawley rats divided into seven group (n=8) and fed for 16 weeks with standard chow (control group), a 60% high fructose diet (Fru group), or the same high fructose diet, but orally supplemented with a low (100 mg/kg BW) or high (200 mg/kg BW) dose of PWE or PEE, or metformin (50 mg/kg BW). Fasting glucose (FG), insulin and blood pressure (BP) were measured every week, and an oral glucose tolerance test (OGTT) performed after 16 weeks to assess glucose tolerance. At the end of the experiment, the levels of triglycerides (TG), total cholesterol (TC), non-esterified free fatty acid (NEFA), superoxide dismutase, catalase, glutathione, glutathione peroxidase lipid peroxidation and DNA strand breakage were assayed. Plasma levels of FG, insulin and BP increased gradually in fructose-fed rats but were markedly lowered by the PWE or PEE. A significant reduction in glycemic response was observed only in PWE following OGTT, when compared with that of Fru group. The plasma level of insulin was also reduced in PWE or PEE rats during OGTT. In addition, the TG, TC, NEFA levels in plasma and liver were also markedly increased in fructose-fed rats but reduced by the PWE or PEE supplementation. Fructose caused oxidative stress and affected the anti-oxidant status including lipid peroxidation, DNA strand breaks and antioxidant enzyme activity. In hepatic tissues, high-fructose treatment also exhibited a 133% increase in comet formation, a 21% increase in lipid peroxidation. Supplemental PWE or PEE significantly (P
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