Anti-fatigue and anti-aging effects of Cistanches tubulosa in senescence accelerated mice
| Autor: | Tsai, Hui-Chun, 蔡慧君 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Cistanche tubulosa is perennial parasite herbs and mainly distributed in warm deserts and arid lands, which is a precious plant for traditional medicine and herbs earned the honor of “Ginseng of the deserts”. Experimental results have demonstrated that Cistanche tubulosa has been shown to have anti-oxidant, and protective effect of liver and brain damage. However, no reference was published for investigating the effect of anti-fatigue. The purpose of this study was to investigate the effects of Cistanche tubulosa on anti-fatigue in senescence-accelerated mice (SAMP8). 3-month-old male SAMP8 mice were used in this study. The mice were randomly divided into four groups, sedentary and exercise were administered by treadmill to control and treatment groups. Groups were divided: sedentary control (SC, n=10), sedentary treatment (ST, n=10), exercise control (ExC, n=10), and exercise treatment (ExT, n=10). The control groups were given double-distilled water (ddH2O) by gavage and the treatment groups were given 200 mg / kg body weight (BW) Cistanche tubulosa extract (CE) by gavage for 4 consecutive weeks. The body weight, food intakes and water consumption were measured. And on the third week, the aging scores were measured. The exercise program was performed after trained 1, 2 and 4 week by all groups, and during exercise that the electric shock frequencies of exercise program were record. The blood glucose and lactic acid concentrations and biochemical parameters of blood were analyzed after the mice sacrificed. And we explored the possible mechanisms in increasing the capability of exercise by glycogen content of muscle and liver. Anti-oxidation state were estimated in blood, liver and brain, malondialdehyde concentration (MDA), glutathione peroxidase actives (GPx) and superoxide dismutase actives (SOD) of blood, and β-amyloid protein (Aβ) deposition of brain were evaluated. The results showed that there were no significant difference in body weight, food intakes and water consumption among groups. The aging score of ExT group was significantly lower than other groups (p < 0.05). Results from the exercise program, during the experiment, at the first, second and fourth week of exercise program that retention frequencies of ExT group was the least. After running, blood lactic acid concentrations of ST and ExT groups were significantly lower than SC and ExC groups (p < 0.05). The content of hepatic and muscle glycogen of ExT group was significantly higher than other groups (p < 0.05). Results from anti-oxidation state of blood showed that the MDA concentration of ExT group was lower than other groups, and GPx activity of ExT group was higher than other groups, especially the SOD activity of ST and ExT groups were significantly higher than SC and ExC groups (p < 0.05). In addition, the anti-oxidation state of liver and brain showed that the MDA concentration of liver and brain in ST and ExT groups were lower than other groups, and the GPx activity of ST and ExT groups were higher than other groups, particularly the GPx activity of brain in ST and ExT groups were significantly higher than SC and ExC groups (p < 0.05). In addition, the β-amyloid protein deposition of brain in ExT group was significantly lower than SC group (p < 0.05). In summary, the supplement of Cistanche tubulosa extract could increase capability of exercise, decrease blood lactic acid after exercise, increase capability of exercise by increasing glycogen content in muscle and liver and improve the antioxidant defense system. There effects show that Cistanche tubulosa extract have anti-fatigue activity. In addition, β-amyloid protein deposition of brain showed Cistanche tubulosa extract was taken benefit of learning and memory ability. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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