Active anti-hyperuricemia ingredients in Phyllantus urinaria Linn. inhibit xanthine oxidase and reduce serum uric acid in rat
| Autor: | Tao, Liang-Yu, 陶良榆 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Hyperuricemia is defined as a serum uric acid level greater than 7.0 mg/dL for males and 6.0 mg/dL for females. In the human body, uric acid is the final product in purine degradation, and xanthine oxidase is the key enzyme in the pathway. In other words, xanthine oxidase is the main enzyme in the pathogenesis of hyperuricemia which may cause gout and other serious diseases such as urolithiasis and nephritis. Phyllanthus urinaria L. (Euphorbiaceae), is widely distributed in tropical and subtropical countries including Taiwan. The plant has been widely used as anti-inflammatory, anti-diarrheal, and hepatoprotective medicines in Asian countries particular in India, China, and Thailand. In our research, P. urinaria extract was used to inhibit xanthine oxidase and control hyperuricemia. The result showed that the ethyl acetate fraction of P. urinaria. extract can efficiently inhibit xanthine oxidase and prevent hypoxanthine from being oxidized to xanthine and further to uric acid. The IC50 of P. urinaria ethyl acetate fraction extract is 22.36 μg/ml (Allopurinol is 1.17 μg/ml). After enzyme inhibition assay, the P. urinaria extract was experimented in an animal model to observe whether it can reduce the serum uric acid or not. After the rats were fed with the extract for 7 days, an anti-hyperuricemia activity in rats which were induced uric acid by subcutaneous injecting the potassium oxonate could be observed. According to the result in animal model, we may consider P. urinaria as a potential treatment for hyperuricemia. Hopefully, we can identify the active ingredient in P. urinaria and obtain the higher efficacy in the animal model. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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