Effects of Cryoprotectant Components on Frozen-Thawed Semen Quality of Alpine Goats
| Autor: | Ting-chieh, Kang, 康定傑 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Sperm cryopreservation is one of the basic technologies in reproduction biology. However, the quality of frozen-thawed goat semen is still not as good as that of the fresh semen. The types and components of cryoprotectants might be the key points for improving the goat semen quality after frozen and thawed. Therefore, the objective of this study is to investigate the effects of concentration of low-density lipoprotein (LDL), amino acid and pH value on frozen-thawed semen quality in Alpine goats. In the following research was to establish the extracting method of LDL from hen’s egg yolk. The results showed that the recovery rate of our extraction method was 60.37%. In the study 1, various concentrations of LDL (between 0 and 20%, w/v) were tested in freezing extenders for Alpine goat semen cryopreservation. The results showed that, the motility (72.65%), survival rate (79.74%), and mitochondrial potential (72.77%) of frozen-thawed Alpine goat semen cryopreserved in extender containing 4% LDL were significantly better than those of other treatment groups and the control group (P < 0.05). In study 2, the experiment 1 was to evaluate the effects of pH value of extender on the quality of frozen-thawed Alpine goat semen. The results showsed that quality of frozen-thawed Alpine goat semen in 4% LDL extender was significantly improved when its pH value was adjusted to 6.4 when compare to that of pH 5.7 and 7.0 (P |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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