Effects of osmotic stress and ischemia/reperfusion injury on the expression of aquaporins in rat intestinal epithelial cells
| Autor: | Shu-Ting Lee, 李淑婷 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Aquaporins (AQPs) are small water-transporting membrane proteins with molecular weights ranging between 25 and 34 kDa. To date, since the initial discovery of AQP1, 13 distinct aquaporins (AQP0-12) have been reported and they are widely expressed in many animal and plant species and in lower organisms. In addition to water transport, various unexpected roles of AQPs such as in the pathophysiology of various diseases were documented in recent years. However, the role of AQPs in the gastrointestinal tract remains largely uncertain. This present study was conducted to explore the effect of hyperosmostic stress and ischemia/reperfusion injury on the expression of AQPs in intestinal epithelial cells. Cultured rat intestinal epithelial cells (IEC-6) were incubated in 300 mM mannitol-containing culture medium or subjected to simulated ischemia/reperfusion treatment for various time periods. The cell viability was evaluated by MTT assay and the expression of AQPs was determined by semiquantitative reverse transcriptase-polymerase chain reaction and Western blotting. Despite reduced cell viability, marked expression of AQP1 and AQP11 proteins was induced in cells exposed to hyperosmotic stress for 16 h while no significant changes in expression levels of AQP3, 4, 7, and 8 were detected. The protein levels of AQP8 and 11 were significantly elevated in cells subjected to ischemic insult for 8 hours, but returned to normal level two hours after reperfusion. The pathophysiological significance for the observed differential expression profiles of AQPs in cells exposed to different stress conditions needs further investigation. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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