Studies of Phalaenopsis Growth Using Carbon Nitrogen Ratio Analysis and Examining the Antioxidant Property
| Autor: | Min-Chuan Chiou, 邱閔絹 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The exploration stalking mechanism of phalaenopsis is important for understaning the plant cultivation and blossom regulation. The goal of this report attempts to find a chemical marker in phalaenopsis to monitor directly the plant growth and blossom. The chemical products are isolated from different orchid cultivars grown with different time periods and examined by elemental analyzer(EA), high performance liquid chromatography(HPLC), and solid state NMR(ssNMR). A sequential of extraction process from different parts of orchid gives three compartment of the methanol phase, the hot-water phase, and the residue. The variation of Carbon/Nitrogen(C/N)ratio, which is measured by elemental analyzer, is considered as an important indication for plant growth and blossom. As a result, a significant variation of C/N ratio was revealed in root(increasing 184%), stem(increasing 179%), 2nd leaf(increasing 270%), and 3rd leaf blade(increasing 274%)after the cooling process for 3 months. Moreover, the longer growth periods of 16-weeks orchid has less variable C/N ratio compared to that of 8-weeks-growth orchid. A methanolic and a hot-water orchid extractions were further subjected to HPLC-Mass spectroscopy attempted to indentify a possible chemical marker. The orchid compartments were further analyzed by C-13 solid state NMR to classify the possible carbon-containing species and their functional groups. The result suggests that the variation of carbon element is less sensitive than the nitrogen element in plant growth. The capability in antioxidant of orchid extract is examined on the extent of the inhibition of Inflammation in zebrafish. A serial of resveratrol derivatives were examined as a control reaction. Consequently, a methanolic orchid extract is demonstrated to possess antiimflammatory effects for sun protection in zebrafish. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|