Homocysteine up-regulates COX-2 expression in Jurkat T lymphocytes.
| Autor: | Chun-Ming Cheng, 陳俊銘 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The aims of this study are to investigate the molecular mechanisms of homocysteine-induce COX-2 expression of T lymphocyte. The effect of homocysteine on T lymphocyte cell line (Jurkat cell) COX-2 expression was examined in vitro. The result from this study may provide insight into the mechanisms contributing to inflammatory response in patients with hyperhomocysteinemia. Experimental results showed that homocysteine can induce T lymphocytes resulting from inflammation, but one of the major regulatory mechanisms is not clear. This study found that the T lymphocytes induced by homocysteine may increase the inflammatory COX-2 gene expression, and induced after 4 hours, COX-2 gene expression had the max expression. Next, further investigate whether hyperhomocystein will induce the increase of intracellular ROS and NO expression, producing the downstream MAPK kinase p38 and JNK phosphorylation and entering nucleus to induce transcription factors NF-κB and Sp1 producing activation, leading COX-2 gene expression, and leading severe inflammation in atherosclerosis. Therefore we believe homocystein play an important role in inducing T lymphocytes inflammatory gene COX-2 expression in atherosclerosis . |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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