A rapid and portable sensor based on protein protected gold nanoparticle probes and lateral flow assay for naked eye detection of mercury ion
| Autor: | Huang, Chung-Chih, 黃崇志 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Recent progress in the laboratory has been a result of improvements in rapid analytical techniques and detection limits for sensing mercury ions (Hg2+) by using the unique optical property of gold nanoparticles (AuNPs). Unfortunately, the contaminated samples are only treated with aqueous solution in the cuvettes and must contain none of salts that could induce the AuNPs aggregation and losing the sensing abilities. Additionally, those experiment procedures for the analysis of metal ion should be operated by the assist of UV-Vis or fluorescence spectrophotometer. A new and simple method for determining the concentration of Hg2+ was developed. We designed the AuNP probes of carboxylic modified protein that could protect the AuNPs from aggregating even in the salinity solutions and employed the lateral flow assay to detect the Hg2+ without complicated instrumentations and technical expertise. The captured AuNP probes on the test zone and control zone of the sensor produced the characteristic red bands, enabling visual discrimination of Hg2+ concentrations. The disposable sensor thus provides a low-cost, convenient, rapid, sensitive, and quantitative tool for the detection of contaminated samples. The demand of Hg2+ analysis in environmental applications requires parallel analytical strategies; such a method with simplicity and rapidity will find wide use in the future. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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