Technologies for protein synthesis using fowlpox virus recombinant
| Autor: | Bor-Sheu Su, 蘇柏栩 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The poultry industry is one of the important livestock industries in Taiwan. One of the major problems faced by the poultry industry is the loss of productivity due to fatal infectious diseases; for instance, infectious bursal disease virus (IBDV), Newcastle disease virus (NDV) and Fowlpox virus (FPV). The main clinical sign of IBDV in chickens younger than two-three weeks of age is immunosuppression resulting from bursal cell necrosis. At three to eight weeks old chickens, IBDV can cause serious clinical symptoms and high mortality. Most protection securities of IBD rely on vaccination. IBDV (VP2) protein can induce host to produce neutralizing antibody; that is why VP2 protein can be an immune protective antigen for disease control. NDV infection usually causes high mortality resulting from acute transmission, so vaccination is a particularly important method to control NDV in poultry. The HN protein of NDV are able to induce neutralizing antibody production for disease prevention. FPV is the biggest and most complicated virus with many advantages for multivalent recombinant vector vaccine development, such as large genome and non-essential genes for expression of foreign antigens. As a safe vehicle, recombinant FPV (rFPV) can prevent not only FPV infection but also diseases of inserted heterologous pathogen genes. Cytokines are kinds of host immunomodulators and great bioactivity adjuvants. Construct with cytokine genes in rFPV will have broad applications in vaccine development by enhancing host immunity. In this experiment, we used FPV as a vector to insert VP2 or HN genes sequences as a multivalent vaccine which can induce chicken immunity against NDV, IBDV and FPV, and co-expressed chicken interleukin-12 (chIL-12) or chIL-18 to increase immunity. The results have shown that rVP2, rHN, rchIL-12 and rchIL-18 synthesized by rFPV have similar bioactivity to that of natural proteins and can protect vaccinated chickens effectively against virulent NDV or IBDV challenge by increasing humoral immunity or cell mediated immunity. This means that rFPV has the potential to be a commercial vaccine which can prevent fatal infectious diseases in poultry. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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