Effects of tryptanthrin on human hepatoma growth in vitro and in vivo.
| Autor: | Chia-Hua Chung, 鍾佳華 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 Hepatocellular carcinoma is a common tumor in Asia and Africa area. Tryptanthrin is a weakly basic alkaloid and has been reported to have various functions, such as anti-microorganisms, anti-parasites, immunomodulatory, anti-inflammatory and anti-tumor activity in vitro. In this study, we examined the efficacy of tryptanthrin in suppression hepatoma growth and further investigated the mechanism by which tryptanthrin alter tumor cell growth. Tryptanthrin significantly inhibited human hepatoma cell proliferation in vitro. However, our data showed that tryptanthrin did not alter expression or activity of caspase proteins, which was different from previous studies on this compound. Tryptanthrin increased intracellular reactive oxygen species and decreased the expression of growth-related proteins. Furthermore, the levels of apoptosis-related protein in the cytoplasmic and nuclear fractions were significantly increased by tryptanthrin treatment. Fluorescence microscope observation further discovered the nuclear translocation of apoptosis-related proteins and their nuclear condensation in tryptanthrin-treated cells. We suggest that tryptanthrin enhanced reactive oxygen species level that led to loss of mitochondrial potential in human hepatoma cells, followed by the activation of apoptotic protein release. The in vivo study subsequently showed that tryptanthrin supplementation inhibited tumor growth in BALB/c nu/nu athymic mice with human hepatoma cell xenograft. Tryptanthrin supplementation increased the number of apoptosis-related protein cells in tumors. The present study suggested that tryptanthrin may have anti-tumor potential in humans. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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