Anti-solvent Precipitation of Zeaxanthin Dipalmitate Particulates from Lycium barbarum Fruits and Effect on Proliferation of Adult Retinal Pigment Epithelium Cells
| Autor: | Jia-Hui Cheng, 鄭家輝 |
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| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 In this study, ultrasonic agitation extraction of zeaxanthin dipalmitate from de-glycosides Lycium barbarum fruits coupled with freeze ethanol anti-solvent precipitation and supercritical anti-solvent(SAS) granulation to produce mirco-sized particulates containing high purity of zeaxanthin dipalmitate .The tetrahydrofuran (THF) extraction of de-glycosides fruits revealed that the content of zeaxanthin dipalmitate is 72.97mg/g and 95.56mg/g, respectively. Subsequently, the freeze ethanol anti-solvent of the THF solution resulted in precipitate containing 824.9mg/g of zeaxanthin dipalmitate with a recovery of 81.9% and supercritical carbon dioxides of the THF solution yield micro-sized particulates containing 925.3mg/g. A three-factor center composited schemed response surface method was finally designed for the SAS granulation to find effects of pressure, feed flow rate and time on precipitate on total yield, purity, recovery and particle size of the zeaxanthin dipalmitate-rich precipitates. Experimental data displayed that the purity of zeaxanthin dipalmitate increased with anti-solvent pressure but the recovery was decreased. The purity also increased with feed flow rate and the recovery present a maximum rate. The SAS precipitates presents micro-sized laminar type morphology and becomes big agglomeration at high anti-solvent time. These purified stable zeaxanthin dipalmitate in aqueous dimethyl sulfoxide solution exhibited anti-oxidation of hydroperoxide damage abilities on proliferating human adult retinal pigment epithelial cells (viability from 22.2% to 31.5%), though it was a meager increase compared to those of zeaxanthin (viability from 43.0% to 93.6%), lutein(viability from 32.1% to 80.1%),b-carotene(viability from 32.1% to 80.3%). |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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