Application of ordered anodic porous alumina in cell culture
| Autor: | Ming-hsin Chen, 陳銘心 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 In this study, highly uniform porous aluminum oxide (Anodic Aluminum Oxide, AAO) film was fabricated by an anodic oxidation process on a surface of aluminum alloy at room temperature. By use of oxalic acid and phosphoric acid as electrolytes respectively, the researcher changed the operational parameters such as electrolyte concentration, anode voltage, and the purity of aluminum substrate in order to control the uniformity of pores and pore sizes while anodization time and temperature were fixed. As a result, the operational conditions with 1M oxalic acid and 40V are optimal to produce highly uniform porous aluminum oxide film. The porous aluminum oxide film was then used for cell culture experiments and MTT cell viability tests. By means of SEM, the researcher observed how cell morphology was influenced by different pore size (20nm-50nm) of aluminum oxide film in 4hr, 1 day, 4 days and 7 days, respectively. The results showed that uniform 50 nm porous aluminum oxide layer could be obtained under the operational conditions of 1M oxalic acid and 40V. As for the cell culture experiments, a better cell morphology could be acquired with the pore size of 120 nm. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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