Study of the Effects of the Yin-Supplement Chinese Herb Di-Huang in Septic Rats
| Autor: | Nai-Lun Chen, 陳乃綸 |
|---|---|
| Rok vydání: | 2011 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 99 The high mortality of sepsis is a difficult issue in clinical practice. It is hard to control in sepsis treatment due to its rapid progression and complicated pathogenesis. In previous study, gender is found to be an important factor of sepsis progression and outcome. Therefore, in this study we tried to adopt the method of sex hormone replacement, in order to explore the relationship among the sex hormones, cytokines and survival rate of the treatment in three consecutive days of Yin-supplement Chinese herb Di-Huang and estradiol before the animal model of sepsis in different gender rats. In this study, we adopt cecal ligation and puncture (CLP) as an animal model of sepsis. 12-week-old adult male and female rats were randomly divided into six groups. Before CLP surgery, the rats accepted 2 ml of low dose (5 mg/ml), high dose (10 mg/ml) Di-Huang by oral administration twice a day and 1 ml of low dose (5 μg/ml), high dose (10 μg/ml) estradiol by subcutaneous injection within three consecutive days. In addition, the control group and sham-operation group. Result: After receiving of estradiol for continuously three days, the concentration of estradiol increased and testosterone decreased, whether in male or female rats. Compared with the control group, the survival rates of sepsis rats were higher and the concentrations of cytokine IL-6 & IL-10 were lower. And after receiving Yin-supplement Chinese herb Di-Huang, the concentration of sex hormones didn’t changed but the survival rate of sepsis rats had a rise trend and there was no effect on cytokine IL-6 & IL-10. According to the result, Yin-supplement Chinese herb Di-Huang could reduce the mortality rate of sepsis rats but the mechanism should be further investigated. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|