Identification of cancer stem cell in esophageal
| Autor: | Wen-Jen Chen, 陳文仁 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 In Taiwan, Esophageal cancer is one of the most common cancer. It ranked 8th leading cause of death in cancer, and ranked 6th of men in Taiwan. In general, the prognosis of esophageal cancer is very poor. The overall five-year survival rate (5YSR) is less than 5%. Many studies have tried to find out the mechanism of carcinogenesis of esophageal carcinoma and set up optimal therapeutic method. Cancer stem cell (CSC) hypothesis is one of the tumor formation models. The general idea of this hypothesis is that there are a little part of cells in tumor, and these cells have the same characteristics with normal stem cell such as self-renew, differentiate, long lifespan, resistant to apoptosis, etc. cancer stem cells are cause clinical treatment obstacles, such as therapy resistance and matastasis. Until now, cancer stem cells have been found in acute myeloid leukemia (AML), breast cancer, brain tumor, lung cancer, liver and gastrointestinal cancer by selection of specific cell surface markers, side population (SP) or tumor sphere. However, these kinds of cells have not been found in esophageal cancer. We isolate CSC through radioresistance, and the ability to form tumor spheres, from three Esophageal cancer cell lines (TE1, TE2 and TE9). Those two methods can increase the probability in identifying CSC. First, we established radioresistance cell lines by irradiated four times sub-lethal dose 8Gy irradiation, then selection cancer stem cell via tumor sphere formation. In tumor sphere formation condition, we can observe that esophageal cancer cells through epithelial-mesenchymal transition, become cancer stem cell and aggregate to a sphere.We then screened surface marker CD44 and CD24 on these sphere cells We inferred that CSC enriched subpopulation in esophageal cancer appeared as CD44-/CD24-. But CD44-/CD24- cells not expressed significant CSC properties compared with differentiated cell-like CD44+/CD24+ subpopulations. Taken together, esophageal cancer cells through epithelial-mesenchymal transition, become cancer stem cell in sphere culture condition. Surface marker CD24, CD44 may not the cancer stem cell markers for esophageal cancer cell lines. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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