Gene expression in the hippocampus of alpha1H T-type calcium channel knockout mice after retrieval of contextual trace fear conditioning
| Autor: | Ni-chun Chung, 鍾妮均 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Voltage-dependent calcium channels (VDCC) trigger many intracellular biochemical events, including muscle contraction, gene expression or hormones and neurotransmitter secretion. Among all VDCCs, the T-type Ca2+ channel α1H subunit encoded by Cav3.2 gene is highly expressed in the hippocampus, which is correlated with contextual learning and spatial memory. Our previously research (Jhe-Wei Shen, 2005) showed that α1H KO mice are impaired in contextual trace fear conditioning, step-down and step-through avoidance tests but functionally normal in locomotor activity and auditory trace fear conditioning. Whether α1H T-type Ca2+ channels are also important to other hippocampal-dependent learning or not is still unknown. In this thesis, α1H KO mice were used to perform Morris water maze and their learning appeared to be normal. Besides, using genome wide microarray analysis for retrieval of contextual fear, asymmetrical gene expression patterns of bilateral hippocampi in training WT groups versus na��ve groups were observed. A cluster of genes that only revealed differential expression in WT group but not in KO group after contextual test may directly modulated by calcium influx via α1H T-type Ca2+ channels. Most of them are functionally related to neuronal activities and neural plasticity. My results showed that α1H KOs, though impaired in contextual fear conditioning, learned as well as WTs on spatial learning. In addition, differentially expressed genes had been screened clustered for retrieval of contextual fear. Some of them could be identified to be modulated by α1H T-type Ca2+ channels. My research indicates that α1H T-type Ca2+ channel independent with formation and retrieval of spatial learning but plays an important role in retrieving contextual trace fear conditioning. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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