Effect Pilocarpine-induced seizure on the GABAergic neurons in dentate gyrus
| Autor: | Hao-Ting Lee, 李皓婷 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Temporal lobe epilepsy (TLE) is the most common epilepsy syndrome with medical refractory characteristics. The specific neuronal loss in the hippocampus is considered to be involved in the development of TLE. Hilus, which is composed of mossy cells and GABAergic interneurons, is a vulnerable areas in the hippocampus after suffering from TLE. Different subtypes of GABAergic interneuron are founds in this area. Among thses subtypes, calretinin-immunoreactive (CR-IR) interneurons which selectively innervate other interneuron are related to epileptogenesis, while neuropeptide Y-immunoreactive (NPY-IR) interneurons exert inhibitory effects on seizure activities. Our aim is to investigate the longitudinal change in the number of the CR-IR and NPY-IR interneurons and the underlying mechanism after seizure onset, immunohistochemistry is used to observe the distribution and the number of these two interneurons between control and epileptic state. Wistar rats were treated with pilocarpine to induce TLE, The alteration was studied from 1-8 weeks after seizure induction. In addition, neurogenesis occurs after seizure induction, therefore we intended to figure out if new-born neuron differentiate into GABAergic interneurons by CR or NPY co-stained with doublecortin (DCX), a marker of new-born neurons Our finding is described as follows: 1) The number of GAD67-IR cells was not significantly changed between control and seizure rats during 1st -8th week. 2) The number of CR-IR neurons was decreased in seizure rats than in control during 1st -8th week after seizure onset. 3) NPY-IR neuron number of seizure rats were more increase than control at the 1st and 8th week, the condition was opposite at the other time point. 4) The number of GAD67+ NPY-IR cells was not significantly changed between control and seizure rats during 1st -8th week. 5) The number of GAD67+ CR-IR cells was decreased in seizure rats than in control during 1st -8th week after seizure onset. 6) At the 8th week, there were DCX+ CR-IR cells and DCX+ NPY-IR cells in the hilus and subgranule zone. In conclusion, we suggest that recurrent seizure development by observing histology in rats brain, it may result from neuropeptide Y and calretinin reduced. Therefore, there was a imbalance condition in hippocampus area and may leading to recurrent seizure. We also found that the new born cell may become the CR-IR and NPY-IR cells, it may be a self-protecting mechanism in the epilepsy. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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