Study on development and effect of recombinant subunits vaccine for type 71 enterovirus
| Autor: | Yun-Ying Lee, 李昀穎 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Because of the high mortality rate of enterovirus 71 (EV71), development of an effective vaccine has become the most important priority in terms of control strategies for prevention. Coat proteins of EV71, VP1-VP3 have been suggested to be the neutralizing epitopes. In this study, mice were immunized with recombinant structural proteins, including VP0, VP1 and VP3, which were expressed by Escherichia coli expression system to produce an effective vaccine for the induction of immunity. The results showed that VP0, VP1 and VP3 protein mixture combined with alum could induce higher EV71-specific humoral and cellular immunity than VP1 combined with alum. Combination of subunit vaccine with alum and TLR ligands such as poly(I:C), CpG and imiquimod can induce a stronger immune response against viral infection. Furthermore, TLR ligands could reduce the Th2 response which was induced by alum. However, immunization of subunit vaccine could not produce neutralizing antibody. Pre-incubation of VP0, VP1 and VP3 protein mixture with anti-virus serum, then applied to neutralization test, also did not inhibit the ability of neutralization of anti-virus serum. Maybe the proteins expressed from E.coli system did not have a good neutralizing epitope. In conclusion, EV71 subunit mixture had a higher immunogenicity than VP1 alone but could not produce effective neutralizing Ab. Poly(I:C) can serve a good adjuvant for EV71 subunit vaccination. Based on this study, the development of effective subunit vaccine for the prevention of EV71 infection is still an important issue. We believe the study might shed the light on further developing effective immunity and also understanding the immune response against EV71 infection. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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