Application of Carbon Nanotube/Chitosan Composite on Nerve Tissue Engineering
| Autor: | Cheng-Lun Chang-Chien, 張簡政倫 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 After peripheral nerve damage, nerve regenerates by re-connecting at both ends of the nerve gap. The tissue engineering for nerve regeneration use nerve conduit as a neural link between the nerve gap that can effectively achieved rehabilitation of neurological function. In recent years, many nerve conduits have been made by chitosan and other polymers, showing good biocompatibility and biodegradability. Due to the structure of carbon nanotube（CNT）, it has unique mechanical and electrical properties. The purpose of this study is to use chitosan and carbon nanotubes mixture as composite material to manufacture a nerve conduit, wish that CNT can enhance the mechanical strength and electrical conductivity of the composite, and also have the biocompatibility and bioresolvability of chitosan. In the experiment, the CNT/chitosan thin film can be fabricate by natural dried, and the fiber can be made by using of hydrodynamic focusing and coaggulation method. Both of them can be seen the nano-scale structure formed by carbon nanotubes from the SEM image. By the physical properties (mechanical and electrical properties) tests, it was found that the mechanical strength and electrical conductivity of CNT/chitosan films were higher than chitosan films, showing that adding CNT can enhance the mechanical strength and electrical conductivity of the composite. The MTT and LDH tests showed CNT/chitosan composite materials do not exhibit cell toxicity. By using oxygen plasma surface modification technology, surface can be modified for more hydrophilic such that water contact angle was reduced to less than 10 degrees, a significant improvement for laminin graft. Furthermore, after the laminin graft, the composite showed enhancement of the affinity of PC12 cells to the material, evidence by the increase of adhesion ratio. The immunofluorescence staining and SEM images results showed PC12 cells can successfully adhere and differentiate on the CNT/chitosan films and fibers; and the PC12 axons are more extended by following the fibers with a directional growth, indicating that these fibers can guide nerve cells to have a role in the growth direction. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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