The moderation of radiotherapy side effects by ethanol extract of red mold dioscorea
| Autor: | Chih-Chen Huang, 黃之丞 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Radiotherapy is frequently used as part of cancer treatment to achieve tumor control. Apart from inducing anti-proliferative and cell-killing effects in tumor tissue, radiotherapy also provokes normal tissue damage such as inflammation and fibrosis. Therefore, how to alleviating the side effects of radiotherapy is a crucial problem. The Monascus species is a Chinese traditional fermentation fungus used on food for over thousands of years in China. The secondary metabolites of Monascus in the contemporary research were confirmed the suppression cholesterol production, anti-inflammation, anti-tumor and suppression tumor metastasis. Accordingly, Monascus metabolites can apply to radiotherapy to alleviate the side effects. In this study, we investigated whether ethanol extract of red mold dioscorea (RMDE) attenuates radiation-induced normal tissue damage in vivo and in vitro. Results showed that oral administration of RMDE can increase animal body weight and food intake but suppress tumor regression. Besides, radiation-induced splenomegaly was significantly alleviated by RMDE. The pro-inflammatory (i.e. tumor necrosis factor-α, TNF-α; interleukin-1β, IL-1β; interleukin-6, IL-6), pro-fibrotic (i.e. transforming growth factor-β1, TGF-β1) and angiogenesis (i.e. vascular endothelial growth factor, VEGF) maker in serum of RMDE treatment group was significantly lower than control group. Furthermore, the results of histopathological slices of lung, liver and kidney in each group did not show any significant difference from histopathological finding. In vitro assays, the cytotoxic assay showed that RMDE have more cytotoxic to B16-F0 cells than skin primary cells. Ankaflavin, monascin and citrinin are non-effect in skin primary cells. Monascin can inhibit B16-F0 proliferation. According to the radiation-induced cell damage model, RMDE and ankaflavin treatment can inhibit TNF-α, IL-1β, IL-6 and TGF-β1 mRNA expression and monascin can inhibit IL-1β, IL-6 and TGF-β1 mRNA expression but not TNF-α. These features of RMDE have made it an attractive candidate to moderate the side effects in clinical radiotherapy. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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