The Preliminary Study of Genetic Structure of Small Abalone (Haliotis diversicolor) in Taiwan
| Autor: | Hsuan-Ming Chang, 張軒銘 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Small abalone (Haliotis diversicolor) is the highest commercial valued aquaculture shellfish species in Taiwan. Recently, the cultivated small abalone production has reduced dramatically to 1% of former peak production. Many researchers have tried to clarify the cause of diseases by investigating pathogens and environmental parameters and have speculated that viruses are to blame for allegedly causing the mass mortality rate. However, there is no consensus yet. Further studies in genetics could be the key in identifying the root cause of the high mortality rate for small abalone production. In this study, we studied the genetic structure of 5 wild and 5 cultured abalone populations collected from Taiwan and Japan. We used 6 microsatellite DNA loci and partial of mitochondrial DNA COI sequences to examine the genetic diversity as well as degree of inbreeding. Among 263 individuals, whose data were obtained from 10 populations and 88 variable sites, partial of mtDNA（mitochondrial）COI sequences（808bp）were categorized into 81 haplotypes. The wild population was the highest in Japan, both in haplotype and nucleotide diversity（JPW, h=0.970; θπ=0.459）. In contrast, the cultured population in Taiwan was the lowest in those two parameters（TWC, h=0.871; θπ=0.316）. A total of 56 individuals from 5 wild populations and 40 individuals from 4 hatcheries（Japan × Taiwan population excluded）were analyzed for six microsatellite loci. Tests for all loci revealed that H-W equilibrium only existed in Taiwan’s Northeast（CE）population, while the results from other populations all deviated significantly from H-W equilibrium. Overall, the observed heterozygosity（Ho）was less than expected heterozygosity（He）of the 9 populations. Allele richness ranged from 33.33 to 23.77（JF, Ar=33.33 ; CP, Ar=23.77）. Results from reconstructed NJ phylogenetic tree, principle component analysis（PCA）and Bayesian method to estimate the genetic components, revealed that Japan wild population, Taiwan wild population and Taiwan cultured population differed in genetic structure, especially the Kaohsiung population（CK）deviated significantly from others. Genetic differentiation index（FST）analysis by mtDNA COI sequence and microsatellite DNA among 10 populations indicated that：among Japan wild populations, there were no differentiation（FST |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|