Pigmentation and pigment regulation in blood parrot cichlid
| Autor: | Yung-Feng Lin, 林永豐 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 The values of ornamental fish depend on size, appearance, color, and pattern. Coloration enhancing could bring more value to ornamental fish. The goals of present study are researching the mechanism of whitening and the impacts of astaxanthin on body color of blood parrot cichlid. The tyrosinase full-length cDNA of blood parrot cichlid was cloned by RT-PCR and RACE method. Analysis of nucleotide sequence revealed that tyrosinase full-length cDNA consists of 1889 bp containing an open reading frame of 1620 bp, which encodes 540 amino acids. There were 5’UTR, epidermal growth factor-like domain, CuA-binding region, CuB-binding region, and 3’UTR in the tyrosinase gene sequence. The expression of the blood parrot cichlid tyrosinase gene, tyrosinase activity, and melanin content decreased during whitening. The retention of intron 2 may be the reason of whitening. The expression of the blood parrot cichlid bad gene decreased during whitening. The expressions of the blood parrot cichlid tyrosinase and bad gene decreased after treated with norepinephrine. Using different sources of astaxanthin to enhance body color of blood parrot cichlid, we found the color of blood parrot cichlid fed with BASF astaxanthin was more identical. Through microscope, red pigment mainly accumulated at skin, scales, and fins. In color enhancing study, all groups had the same tendency: L* value decreased, a* value increased, and b* increased. In red blood parrot cichlid, astaxanthin, canthaxanthin, and β-carotene could be detected by TLC. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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