The Studies of Ant Venom Allergen Phospholipase A1 in Taiwan (Formicidae：Solenopsis invicta、Solenopsis geminata、Leptogenys kitteli、Pachycondyla javana、Odontomachus monticola)
| Autor: | Huang Chia- Ming, 黃嘉銘 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 The ant is evolved from a Hymenoptera Vespoidea. Lay ovipositor, aculeate and surrounding tissue turn to venom gland for survived. The red imported fire ant (RIFA) and tropical fire ant (TFA) are exotic species in Taiwan, they are aggressive, have a painful and allergenic sting. RIFA is distributed in the north of Taiwan at present. TFA is distributed in the south of Taiwan. Leptogenys kitteli, Odontomachus monticola and Pachycondyla javana are native in Taiwan. Workers easy to respond defensively to nest disturbance. The poison protein in the venom composition is the main cause of the allergy. Sol i 1 and Phospholipase A1 are the same poison protein composition, and the amino acid sequence has already been determined. In this studies, ant's venom glands were dissected under the microscope and we finds five kinds of ants have a lot of the cells in the filamentous gland, convoluted gland and sting apparatus. And cell is distributed on the poison sac. surface, but the Odontomachus monticola does not this discovery. It’s to be aimed at Sol i 1 amino acid sequence to synthesize antigen, and make the polyclonal antibody from rabbit. Use Western blot analysis and Liquid Chromatography –Mass Spectrometry Analysis to investigate two fire ants species in Taiwan, with three Ponerinae ants comparing the poison protein and COI sequence Phylogenesis, and proved the antibody of Sol i 1 can binding forms the similar protein. Pachycondyla javana may to cross- react with RIFA and TFA phylogeny relationship between these Hymenoptera insects and RIFA is closer, the their antigen binding site of Phospholipase A1 would be more similar. The similar of these antigen binding site may inereaoe the probability of the cross reactivity. Analyse via the IV protein composition. There are 5 base of TFA protein Sol g 1 with Sol i 1 amino acid sequence. And the sequence coverage is 20 %, so it confirmed to be similar Phospholipase A1. Perhaps the Pachycondyla javana protein Pjav02 is similar Phospholipase A1, but because pressed for self-same amino acid sequence, so undeterminable. Sol g 1 and Pjav02 can be identified by Sol i 1 antibody, because both of them have the 50 % similar amino acid sequence of the Sol i 1 compose antigen sequence. Pass the research of this thesis so the Sol i 1 antibody can screened the Phospholipase A1 for other ants species. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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