Bulb Cultivation and Flowering Characteristic of Oriental Lily
| Autor: | Wen-Hsin Chang, 張文欣 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Lilium speciosum Thunb. evolves variations due to geographic isolation, and distribution in Taiwan and China. We cultivated scales of native L. speciosum Thunb. collected from Taiwan, Japan and China in vitro for reproduction of regenerated bulblets, which were further cultivation in the phytotron (20/15°C) for records of growth characteristics. The status of scale-leaves of L. speciosum Thunb. var. gloriosoides Baker derived from Taiwan was the best and applicable to production of high quality plantlets ; the growth of L. speciosum Thunb. var. gloriosoides Baker derived from China preferring bulbs to scale-leaves was applicably cultivated to excellent bulbs via cold storage bulbs. And the plantlet with the scale-leaves and bulb of L. speciosum Thunb derived from Japan. With scales derived from L. speciosum Thunb. var. gloriosoides Baker’s bulblets that have been cultivated in vitro, the bulblets cultivated from these scales for 2 months were transplanted to MS culture media containing sucrose with different concentrations. After three months culture, the maximum 4.3 scale-leaves were observed on one plantlet processed in the 30 g l-1 sucrose ; the bulb’s heaviest fresh weight, 0.4 g, was obtained in the 60 g l-1 sucrose. For transplantation, the plantlets reproduced from scales were transplanted through two methods such as directly transplanted plantlets and cold storage bulbs. With plantlets of L. speciosum Thunb. var. gloriosoides Baker having cultivated in the greenhouse for seven months, the diameters of bulbs processed in both 60 g l-1 and 90 g l-1 sucrose were larger than those in other sucrose and the percentage of sprouting for bulbs processed in the 90 g l-1 sucrose was the highest, 46%. Lilium speciosum Thunb. var. gloriosoides Baker is a late-flowing plant due to high temperature or a short low-temperature period in winter. We planted bulbs with circumferences of 10-18 cm in the greenhouse’s pots, and then transplanted them with buds exposed into phytotrons (30/25, 25/20, and 20/15°C). After planted in an environment (30/25°C) for six weeks, the buds had their diameters rapidly increased and the pedicels extensively elongating over time that demonstrated fast-growing pedicels of plants processed in this environment (30/25°C). Plants processed at 30/25°C, first buds transformed to be in full bloom average number of day 184.3. Plants processed at 25/20°C, first buds transformed to be in full bloom average number of day192.7. Plants processed at 20/15°C had their buds growing slowly. In the experiment for pollen cultivated in vitro, the pollen growing at 20/15°C germinated at the temperature of 30、25 and 20°C, the percentage of germination were 4, 13 and 30, respectively. Then to improve the quality of cut flowers from riental lily, we took oriental hybrid lily, ‘Medusa’ and ‘Marco Polo’, for differentiation of flower bud and flowering. ‘Medusa’ was planted in boxes to observe differentiation of flower buds, development of floral organs, and vase life of flowering. With primordium having grown originally, the plants completed outer perianth totally developed in week 2, 70% inner perianth developed as well as 30% stamen formed in week 3, and pistil totally developed in week 4. Pedicels extended abruptly between week 8 and week 9 ; flower buds elongated in week 10 ; flowers harvested due to colors changed had the vase life of 13 days. ‘Marco Polo’ with circumferences from 14 to 16cm was dipped in different concentrations of gibberellin, and processed over different periods for later planting ; flower buds growing from bulbs of ‘Marco Polo’ which were dipped in 200 mg l-1 and 300 mg l-1 gibberellin for 10 minutes tended to elongate. |
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