Effects of different extenders and storage methods on caprine spermatozoa during non-breeding season

Autor: Chung-Yu Cheng, 鄭淳予
Rok vydání: 2010
Druh dokumentu: 學位論文 ; thesis
Popis: 98
The purpose of this research is to compare the effect of different storage method and extenders on goats spermatozoa. Two trained mature male LaMancha goats were chosen to collect the semen by artificial vagina (AV), and the semen were pooled, 12 ejaculations were collected in non-breeding seasons. Each pooled semen were separated into two treatments, with or without seminal plasma semen. In addition, different volumes of egg yolk or skimmed milk extenders were added to the pooled semen depend on the concentrations of sperm. Second part of extenders (including glycerol) were added to the semen after 120 minutes of balanced. After adding second part of extenders, semen samples were kept at -196℃ and 4℃ for preservation. The sperm motility scale (SMS), viability (VIA), and acrosome integrity (AIR) of semen samples kept at 4℃ were evaluated respectively for a week. The result of this research: The SMS, VIA (%) and AIR (%) of goats semen without seminal plasma diluted with egg yolk extender (EYE) for storage 1 week at 4℃ were significantly higher than those of goats semen with seminal plasma(2.72 ± 0.14 vs. 1.37 ± 0.18;72.33 ± 0.03 vs. 29.01 ± 0.04;92.97 ± 0.03 vs. 42.86 ± 0.04). And the SMS, VIA (%) and AIR (%) of goats semen with or without seminal plasma diluted with skimmed milk extender (SKM) for storage 1 week at 4℃ were no significantly difference. The acrosome integrity of goats semen without seminal plasma diluted with EYE and preserved under 4℃ were higher than SME(92.97 ± 0.01% vs. 76.89 ± 0.01%) (P<0.05). The goat semen with seminal plasma diluted with SME and storage under 4℃, had a better effect on all parameters(P<0.05). In cryopreservation, there was no significant difference between extenders on all parameters. The SMS and VIA (%) of semens without seminal plasma were higher than those of semen with seminal plasma (2.60 ± 0.07 vs. 1.98 ± 0.07;32.74 ± 0.02 vs. 19.44 ± 0.02 ). The SMS, VIA (%) and AIR (%) of goats semen equilibrated over 90 minutes after added second part of extender were higher than those without equilibration .In summary, goat semen diluted with EYE and equilibrated over 90 minutes after added second part of extender could get good cryopreservation results in this research. Eight does synchronized were chosen for artificial insemination. Fecal progesterone were detected for pregnancy diagnosis after 19 days of artificial insemination. The result showed that 4 does were pregnant, which 3 of SME, and 1 of EYE.
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