Potential application and health benefit of a novel probiotic-biocellulosic microsphere
| Autor: | Wen-Ling Lo, 羅文琳 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 The present study was to produce a probiotic-biocellulosic microsphere using Gluconacetobacter xylinus and evaluate its potential intestinal health-enhancement ability. The results demonstrated that the desired stirring speed for the production of biocellulosic microsphere was 400 rpm, hence to obtain microsphere samples of desired shape and size in this study. The yield and diameter of the biocellulosic microspheres were significantly increased (p ＜ 0.05) as the cultivation time increased. No significant difference in the number of microspheres was observed among different cultivation conditions. The yield of microsphere after 2 days of cultivation (regarded as one basic cycle) was lower than those of 4, 6 and 8 days. Since the yields of microsphere after 2, 3 and 4 cycles (with 2 days per cycle) were higher than those obtained in 4, 6 and 8 days (43.2%, 56.4% and 64.0%, respectively). The biocellulosic microsphere after 2 days of cultivation could produce a desirable spherical shape. Therefore, the cultivation conditions (e.g. temperature at 30°C, stirring speed at 400 rpm and 2 days of cultivation) were selected to produce microsphhere samples for the following in vivo and in vitro experiments. The probiotic-biocellulosic microsphere was then prepared by cultivating the microsphere in a culture solution of Bifidobacterium bifidum. The observation using a scanning electron microscopic has revealed the presence of B. bifidum inside the cellulosic network of microshpere, suggesting the ability of microsphere in carrying B. bifidum. In vitro study showed that the maximum release of B. bifidum from the probiotic-biocellulosic microsphere into the MRS broth was achieved at 60 min. It was revealed that the probiotic-biocellulosic microsphere was found to be able to carry and release the B. bifidum. Further in vivo study has demonstrated that the administration of the probiotic-biocellulosic microsphere could significantly (p ＜ 0.05) increase the number of fecal lactic acid bacteria and fecal moisture content as well as lower the fecal pH. These results suggested that the consumption of the probiotic-filled biocellulosic microsphere might exert a favorable effect on improving intestinal health, and be explored as a new fiber-rich food for functional food development. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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