Study on the Effects and Mechanisms of Vitamin C, Selenium and Chondroitin Sulfate on Osteoarthritis
| Autor: | Tzu-Ching Huang, 黃姿菁 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Osteoarthritis（OA） is an age-related degenerative joint disease which characterizes by the loss of cartilage extracellular matrix and results in chondrocytes’ death and cartilage damage. Vitamin C and selenium (Se), a trace mineral element, are essential nutrients for people to maintain health, however, it is still not known whether they are protective against OA or not. The mono-iodoacetate（MIA）has been known to induce OA in rats. In this study, we designed a series of experiments to figure out the effects and mechanisms of vitamin C and Se on the protection against the MIA-damaged SW-1353 cells. In addition, we also explore chondroitin sulfate sodium salt (CS), a health food, on improving abrasion of articular cartilage. Over-production of reactive oxygen species (ROS) is one of the major causes of OA; thus, increase in anti-oxidative capacity to remove ROS could lessen the severity of this disease. Transcription factor of nuclear factor-erythroid 2-related factor 2（Nrf2） is responsible for regulating a variety of antioxidant enzymes’ expressions. Our results showed vitamin C and Se could increase Nrf2 expression which was decreased in the MIA-treated SW-1353 cells. Accordingly, we proposed that the anti-oxidative capacity of MIA-treated SW-1353 cells might be enhanced after vitamin C and Se additions. Moreover, vitamin C and Se additions could decrease the MIA-induced SW-1353 cell’s apoptosis by activating NF-κB and p53. The expressions of apoptotic proteins, such as Bax and cytochrome c, and the activations of pro-caspase 3 and pro-caspase 9 were reduced. In order to maintain genomic stability, there are several DNA repair systems are activated as DNA is damaged. Interestingly, we also found the expressions of DNA repair-related proteins including Ataxia telangiectasia mutated (ATM) and Nibrin (NBS1) were also increased after vitamin C and Se treatment. In a MIA-induced OA model of rat, the pathological results showed CS would lessen the stress of chondrocytes and improve abrasion of articular cartilage. In Conclusion, This study demonstrates that either vitamin C, Se or CS is protective against OA progression. It seems worthwhile to further evaluate their clinical efficacy. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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