Effect of Morus alba L. Extracts on Cancer Metastasis
| Autor: | Chin-Yun Cheng, 鄭錦雲 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 The major cause of cancer mortality is cancer metastasis. If metastasis is well inhibited, patient’s life will be extended. Recently, cancer treatment with natural products is more popular. However, most of these treatments have been aimed at killing cancer cells. Inhibition of cancer metastasis may provide an alternative therapeutic strategy. Fibronectin matrix [polymeric fibronectin (polyFN)] assembled on suspended cancer cells has been identified as an essential ligand responsible for lung endothelial colonization followed by final metastasis and thus is a potential target for natural products with anti-metastatic ability. Resveratrol is one of compositions in Morus alba L. extracts and has been reported to have anti-metastatic effect. However, its exact inhibitory mechanism is not well understood. We found that Resveratrol interupted polyFN assembly on cancer cell surfaces and thus prevented cancer metastasis in the lungs. Next, we extracted the Morus alba L. by using different temperatures and extraction solvents during sonication for testing the inhibitory effects of these extracts on polyFN assembly of melanoma cells. We found that the inhibitory effect of extract A ( 50% alcohol at 25℃ for 1 hour) was higher than that of extract B (50% alcohol at 25℃for 2 hours) and extract C (H2O at 60℃for 1 hour),.The level of this inhibition was comparable to that of Resveratrol. The concentration of was However, determined with HPLC, the concentration of Resveratrol in extract Awas much lower than that used to inhibit lung metastasis of melanoma cells, suggesting that it was other yet-to-be identified components responsible for the anti-metastatic effect of Morus alba L. extractA.Therefore, We employed GC/MS and identified 6 candidate compounds. Conversely, two out of these 6 compounds, Palmitic acid and Furfural, promoted polyFN assembly on melanoma cells. These results suggest that the main component in extract A responsible for the anti-metastatic effect is yet to be identified. Our future work is to further analyze the composition of extract A with LC/MS and try to ultimately identify the true effective components using distinct selection criteria. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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