Study on isolation processes of bioactive flavonol glycosides from Taiwan endemic plant Cinnamomum osmophloeum leaves
| Autor: | Wei-chien Husng, 黃偉健 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Cinnamomum osmophloeum Kaneh. (Lauraceae) is an indigenous tree that grows in Taiwan’s natural hart wood forest at an elevation between 400-1500 m. Traditionally, the leaves of C. osmophloeum are used in folk medicine for the treatment of inflammation, intestinal infections, astringent, diuretic and diabetic complications in Taiwan. Previously our research group have been reported the anti-inflammatory and anti-diabetic flavonoid (kaempferol glycosides) from the leaves of this species. In the present study, a capillary electrophoresis quantification method, using catechin as the internal standard was developed for the analysis of kaempferitrin (C1), kaempferol 3-O-β-D-glucopyranosyl -(1-4)-α-L-rhamnopyranosyl-7-O-α-L-rhamnopyranoside (C2), and kaem pferol 3-O-β-D-apiofuranosyl-(1-2)-α-L-arabinofuranosyl-7-O-α-L-rham nopyranoside (C3). Optimal conditions found were 35 mM sodium borate buffer, pH 9.8, separation voltage 25 kV, hydrodynamic injection time 5s and temperature 25 ºC. Linearity was found over the 0.075–0.6 mg/mL concentration ranges of each compound (C1, C2 and C3) analyzed. The developed method has been applied for determination of C1, C2 and C3 in a series of C. osmophloeum leaves. On the other hand, we also developed a simple extraction process using salt water (0.1 M NaCl) for the separation of C1, C2 and C3 from C. osmophloeum leaves. The air-dried powder of C. osmophloeum leaves was extracted for 2 h with 0.1 M NaCl under stirring at room temperature. The extract was filtered and separated into two parts. In the first part the NaCl was removed using electro dialysis and subsequently introduced into a macroporous adsorption resin, where as the second part directly (without NaCl remove) introduced to adsorption resin for recovering the C1, C2 and C3. The adsorption was performed on Diaion HP-20 resin packed in a glass column (L 31 cm × OD 3.3 cm, with 36 g resin), and desorption was carried out using 95% ethanol. Through the process, in the first part the recoveries of C1, C2, and C3 were 95.36%, 92.21%, and 96.39%; whereas the same in the second part were 94.60%, 94.72%, and 97.81%, respectively. The content of C1, C2, and C3 in the above both parts were quantitatively determined using capillary electrophoresis and found the results was comparable. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|