Part I Morus alba leaves extract prevents atherosclerosis developmentPartII Inhibitory effects of Nelumbo Nucifera leaf extract on human breast carcinoma MCF-7 cells
| Autor: | Mon-Yuan, 楊孟元 |
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| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 Part I Cardiovascular disease has been a major cause of mortality in human and hyperlipidemia plays an important role contributing to atherosclerosis. Oxidated LDL (ox-LDL) has known to damage the endothelial cells and cause endothelial dysfunction. And ox-LDL-mediated foam cell formation by macrophage initiates the cascade of atherosclerosis. This study was to evaluate the anti-oxidative and anti-atherosclerotic effects of the MLE and MLPE by a model of in vitro antioxdation and cell culture. As shown in previous studies Morus alba L. leaves extract is a nature plant containing a lot of pigments that was found to possess antioxidative activity. In this study, we evaluated the antioxidative activity of the Morus alba L. leaves extract (MLE and MLPE) by measuring their effects on LDL oxidation and anti-atherosclerotic abilities. MLE and MLPE possessed the ability of DPPH radical scavenging. The antioxidative activity of the MLE and MLPE on LDL oxidation was defined by relative electrophoretic mobility (REM) and thiobarbituric acid-relative substances (TBARS) assay. Our results showed that MLE and MLPE were able to reduce REM and inhibit TBARS assay in the Cu2+-mediated oxidative LDL. Taken together, low concentration of MLE and MLPE could reduce marked the cellular cholesterol and triglycerides accumulation in macrophage and inhibit the formation of foam cells. MLE inhibits ox-LDL-induced CD36 and SR-A expression. We found MLE also reduced ox-LDL-mediated ROS increased. MLE reduces macrophage phagocytosis by Dil-acLDL. Our study demonstrates the MLE and MLPE can inhibit the oxidation of LDL and has the anti-oxidatic and anti-atherosclerotic properties. Part II Over the pass decade, advances in understanding carcinogenesis have made possible the identification of the candidates of chemopreventive agents that are being developed to hit the key molecular targets. Nuciferine Leaf extract (NLE) has been identified a lot of benefit about anti-oxidant and reduced risks of cardiovascular disease. In this study aimed to define the anti-carcinogenesis and the mechanism of NLE reduced chemical-induced cancer in animal model. First we found that NLE extracted from Nuciferine Leaf suppressed the chemical induced incidence, multiplicity and tumor volume of mammary tumors in rats. Next, we observed that NLE caused the cell cycle arrest in the G0/G1 phase in human breast cancer cell line MCF7. The result also revealed that NLE reduced phosphorylation in Akt and expression of fatty acid synthase (FAS) in NLE-treated MCF7 cells. Finally, murine xenograft model also confirmed that NLE inhibited tumor growth rate and tumor weight. Altogether, the findings of the study reveal that NLE should be used to develop a new chemopreventive agent. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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