Using Drosophila Model to Screen Potential Anti-Aging Dietary Nutritional Supplements and Chinese Herbal Medicines
| Autor: | Ya-Jung Lu, 呂雅榕 |
|---|---|
| Rok vydání: | 2010 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 98 The natural phytoalexin resveratrol, found in grapes and red wine, has been proven effective in prolonging lifespan of yeasts, worms, and flies. In this study, we screened several potential antioxidative dietary nutritional supplements and Chinese herbal medicines (CHMs) for improving lifespan in the Drosophila model. We had analyzed the screening of several dietary nutritional supplements and CHMs with antioxidative property, feeding effective concentration for Drosophila, and testing about climbing ability, sexual behavior, measurement of lipid peroxidation (LPO) level, oxidative stress treatment (such as feeding paraquat and H2O2 in this study), anti-oxidative enzyme activity assay (such as activity of total Superoxide Dismutase (SOD) and activity of catalase) in wild-type (CS) and aging-related mutant E2F Drosophila. Data were analyzed the Log-rank test and Student’s t-test, p < 0.05 was considered statistically significant. The results showed that curcumin and Salviae miltiorrhiza (Dan-Shen) could prolong its 50% survival and mean lifespan (p < 0.05). Since these dietary nutritional supplements and CHMs exhibited strong antioxidative property, preventing oxidative stress as well as lipid peroxidation might be the possible mechanism involved in prolonging lifespan in Drosophila. Our study revealed that curcumin and Salvia miltiorrhiza might have the potential to anti-aging and life-expectancy, indicating that some antioxidative dietary nutritional supplements and Chinese herbal medicines may be used as the initial measure for anti-aging study in flies model. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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