Improvement of the sensitivity of surface plasmon resonance biosensors based on phase detection techniques and the development toward
| Autor: | Ya-Jung Wang, 王雅榕 |
|---|---|
| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Surface plasmon resonance (SPR) biosensor has high sensitivity for detecting refractive index changes of the samples. Using 532nm green laser as light source based on a special phase detection technique provides a wide dynamic range, better resolution of image spatial resolution, and good sensitivity. In this study, we proposed a common-path method to reduce the inaccuracy of the phase analysis due to the optical path difference and the disturbance from the external environment. The use of sucrose solution validates the resolution of our system for small changes in refractive index with wide dynamic range. The resolution of our system for the refractive index can be improved down to 2×10P-6P RI. Then we combine the improved SPR biosensor with a CCD video camera to form a SPR image system that could measure the spatial distribution of refractive index in the image, and the best resolution of the image system for refractive index could reach 4×10P-6P RI. Since there are still some errors in the phase analysis of the image system, we hope that we could overcome the factors of errors and improve the resolution of the image system, and then use the SPR image system to investigate the influence of environmental changes on microorganism. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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