Production of antioxidative activity compounds from shiitake stipes by Bacillus subtilis CW-116
| Autor: | Ko Chun Hung, 葛俊宏 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 The shiitake stipes was used in this project as a nutritional source for the production of antioxidants from Bacillus subtilis CW-116, and the effects of different culture conditions on antioxidant production were investigated. DPPH free radical scavenging activity, ferrous ion-chelating ability, reducing ability, and ABTS free radical scavenging activity (Trolox Equivalent Antioxidant Capacity assay, TEAC) were measured in order to evaluate antioxidant activity. The effects of different culture times, concentrations, and volumes were compared in order to obtain the optimal culture conditions. To further investigate the physical and chemical properties of the active components, the fermentation broth was further separated and purified using chromatofocusing and ammonium sulfate precipitation, and the isolated active components were then analyzed to determine the antioxidant activity. The results showed that the antioxidant activity of B. subtilis CW-116 was significantly enhanced when cultured with shiitake stipes. The optimal culture conditions for different antioxidant activities were as follows: at a culture time of three days, concentration of 4%, and volume of 75mL, the culture exhibited the highest DPPH free radical scavenging activity of 88.34±0.54 (trolox eq mg/g sample) (p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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