Effects of Different Exercise Modes onPharmacokinetics of Ferulic acid
| Autor: | Chiu-Fang Lee, 李秋芳 |
|---|---|
| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Ferulic acid is an active constituent found in the root of Angelica sinesis Diels. Several studies have reported that ferulic acid possesses various pharmaceutical activites, including anti-sports-fatige, anti-inflammatory, anti-oxidation and the prevention of atherosclerosis. The present study is to investigate the effects of different modes of exercise on pharmacokinetics of ferulic acid. A sensitive and simple HPLC method for determination of ferulic acid in rat plasma was established and validated. A Hitach L-2130 HPLC pump system equipped with a photodiode array detector was used to analyze the ferulic acid on a C18 reversed-phase column at 260 and 320 nm. Methyl paraben was applied as the internal standard. A mixture of 65% acetic acid glacial buffer and 35% methanol was used as mobile phase at a flow rate of 1 mL/min and an injection volume of 50 μl. The calibration curve is linear at the concentration ranged from 0.05 ~ 5 μg/mL. The correlation coefficient of the linear regression analysis was higher than 0.9999. The intra day and inter day relative standard deviations (R.S.D.%) were below 5%. The analytical recovery is higher than 85%. The established HPLC method was further applied for investigating effects of different exercise modes (sedentary, running and swimming) on pharmacokinetics of ferulic acid in rats. After training for one month, the rats were subjected to oral administration (single dose) of extract of Angelica sinesis Diels. The pharmacokinetic of ferulic acid was well described by a one compartment model. Area under the curve (AUC) of running group was significantly higher than sedentary group. Clearance (Cl_F) of running group was significantly lower than sedentary group. In addition to AUC and CL_F, there were no significant differences on other pharmacokinetic parameters among three groups. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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