Expression and promoter analyses of Arabidopsis polyubiquitin genes, UBQ10, UBQ11 and UBQ14
| Autor: | Shu-Chih Chang, 張淑芝 |
|---|---|
| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Ubiquitin (Ub) is a conserved 76-amino acid protein among all eukaryotic organisms. According to previous genetic and molecular studies, the majority of cellular Ub molecules in Arabidopsis are encoded by five polyubiquitin genes. These five genes can be further divided into two subtypes based on the gene structures phylogenic analyses. In this study, we had focused on transcription regulation of the second subtype, UBQ10/UBQ11/UBQ14. The promoter deletion and transient analyses indicated that the positive regulatory regions representing locations between -2044 to -1849, -577 to -258, and -714 to -636 were defined on the UBQ10, UBQ11, UBQ14 promoter sequences, respectively. Besides that, a negative regulatory region (-798 to -577) was also defined on UBQ11 promoter sequence. The promoter sequences of these genes were furthered analyzed by MEME software, and the results indicated three conserved cis-acting elements ubiquitin promoter elements (UPEs). We also compared the expression patterns of UBQ10/UBQ11/UBQ14 during various developmental stages and under different temperatures and light treatments. The results showed that UBQ14 and UBQ11 had a higher expression in low temperature. Moreover, the transcription level of UBQ14 also decreased transcription level under UV-B and dark treatment. In contrast, UBQ10 expression remained constantly under various environmental treatments. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|