Effect of melatonin on the integrity of blood brain barrier in permanent stroke model in mice
| Autor: | Li-Der Chang, 張立德 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Cerebral ischemia stroke can cause damage to the neurovascular unit and formation of brain edema. Melatonin (N-aceyl-5-methoxytryptamine) is a well-known, potent free radical scavenger and an antioxidant. Therefore, a series of experiments with delayed treatment of melatonin have been employed to examine whether exogenous melatonin offers neuroprotective action against middle cerebral artery occlusion (MCAo). In this study, adult male ICR mice were subjected to MCA ligation. Melatonin (5 mg/kg) or vehicle were given before MCA ligation. Brains were sectioned and stained using Nissl stain. Then, software was used to calculate infarction size, brain edema and cell count. 1% Evans Blue was injected after MCA ligation to observe the integrity of blood- brain barrier (BBB). We used neurobehavioral outcome measures to evaluate the ability of motor and sense. The protein expression of aquaporin-4 (AQP-4), Matrix metalloproteinases-9 (MMP-9) and Tight junction protein were determined by Western blotting at 24 hours of ligation. Treatment with melatonin reduced the infarction size and brain swelling after middle cerebral artery ligation. Our results indicate that the administration of melatonin reduced the size of cortical and infarction and brain edema after ischemia. Treatment with melatonin can protect the compromised structure of BBB after MCAO. The decrease of expression of aquaporin-4 may be associated with brain edema after treatment with melatonin. Our studies suggest that melatonin-treated animals not only have reduced brain damage but also decreased brain vasogenic edema after ischemia. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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