Improvement of Artificial Skin
| Autor: | Hao-Wei Su, 蘇浩薇 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 In this work, “Dielectrophoresis(DEP) Method” was employed to form Carbon nanotube (CNT) embedded substrate. The characteristics、topography and nanomechchamical properties of Collagen on ITO were investigated. With Atomic Force Microscope(AFM), the stress and the adhesion forces of CNTs embedded Collagen/ITO substrate have been discussed and measured. Collagen was chosen to be as a testing film .An film on ITO(Indium Tin Oxide)、Mica and PDMS(Polydimethylsiloxane) at different of temperatures and concentrations were revealed by AFM(Atomic Force Microscope). The NIH-3T3 Cells were then cell-cultured on substrates to test the bio-compatibility of different substrates. Our results have demonstrate that the CNT embedded Collagen/ITO is compatible for bio-samples. PART one and PART two: We chose Collagen as a testing film .Then we put it on different substrate at different of temperatures and concentration .The topography and adhesion force of Collagen on different substrates at temperatures (4℃,26℃) with different concentration(0.135μg/ml、1.35μg/ml、13.5μg/ml) were investigated by AFM. PART three and PRAT four: We embedded CNTs into collagen film on the substrate to change the mechanical properties of the substrate. Our results demonstrate that CNTs is effective to change the stress of the substrate.NIH-3T3 was chosen to check the biocompatibility of CNTs/Collagen substrate. The NIH-3T3 on the CNTs/Collagen substrate were observed in vitro by optical microscope. We find NIH-3T3 Cells could proliferate on CNTs embedded collagen/ITO substrate. PART five: A comparison of cell proliferate was proposed between CNTs embedded collagen/substrate. The resulted demonstrated that collagen is a better bio-compatible film for bio samples. In this work, the characteristics including topography and nanomechamical properties of collagen on ITO(Indium Tin Oxide),Mica and PDMS() at different temperature (4℃,26℃) were revealed by AFM. It was been found that collagen has better nature on PDMS rather then on ITO and an Mica. Moreover, “Dielectrophoresis(DEP) Method” was employed to form Carbon nanotube (CNT) embedded substrate, then the characteristics、topography and nanomechchamical properties of Collagen on ITO were investigated, then the NIH-3T3 Cells were then cell-cultured on substrates to test the bio-compatibility of different substrates and Our results have demonstrate that the CNT embedded Collagen/ITO is compatible for bio-samples. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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