Using proteomic approaches to identify the differential expressed proteins in the plasma of patients with Parkinson's Disease
| Autor: | Chiang, Chia-ying, 蔣佳穎 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Parkinson's disease (PD) is a degenerative disorder of the central nervous system that often impairs the patient’s motor skills, speech, and other functions. The diagnosis is based solely on medical history and neurological examination conducted by interviewing and observing the patient in person using the Unified Parkinson's Disease Rating Scale. Currently, there is no convenient clinical test for PD and specific plasma PD markers are needed in urgent. In this study, we employed proteomic techniques to dissect possible plasma protein markers for PD. By using immobilized metal affinity chromatography and two-dimensional electrophoresis, we identified dozens of differentially expressed or modified phosphoproteins which might be potential PD plasma markers. Most notably, the phosphorylation status of alpha 2-HS-glycoprotein and alpha,beta,gamma firbirnogen/fibrin were respectively up-regualted and down-regulated in the plasma of PD patients, but not healthy individuals. On the other hand, an immunoglobulin (Ig) profiling method was established and used to reveal the specific plasma Ig repertoire related to PD. Interestingly, two apparent IgG hybridization signals corresponding to the 50 and 70 kDa E.Coli proteins were generally observed in the Ig profiling of PD patients. Results present in this study provide potential plasma protein markers that can be used for developing a clinical test for PD. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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