Efficiency Enhancement of DNA Hybridization by Pre-Hybridization on Microarrayed Gene Probes
| Autor: | Kai-Ren Jiang, 姜凱仁 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 The effect of pre-hybridizing genetic DNA target on the hybridization efficiency of the second target samples is studied on two probe configurations and various probe concentrations. Three sets of microarrayed DNA probes and their complementary samples are utilized. Each set contains a pair of DNA probes and a pair of their complementary samples labeled with two distinct fluorescent dyes. The sequence of each probe is especially designed so that two targets are simultaneously complementary to two adjacent sections of the probe. The molecular steric effect on the hybridization efficiency is investigated by comparing the dye signals between configurations of one-target and two-target hybridization scenarios. The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe. The first DNA target is also found to sacrifice signal intensity for the second one. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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