Human placenta-derived mesenchymal cells (hPDMCs) seeded in alginate-based nanocomposites combined with biodegradable precision scaffolds for cartilage tissue engineering
| Autor: | Shun-Jung Cheng, 鄭舜榮 |
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| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 Human mesenchymal stem cells are studied extensively such as bone marrow mesenchymal stem cells (BMSCs). However, mesenchymal stem cells from bone marrow could be limited. Human term placenta, a temporary organ is discarded postpartum. In this study, we used human placenta-derived mesenchymal cells (hPDMCs) seeded into PLGA precision scaffolds for chondrogenesis. The mixture, cellulose binding domain Arginine-Glycine- Aspartate (CBD-RGD)/nano-calcium deficient hydroxyapatite (nCDHA) which was absorbed transforming growth factor beta-3(TGF-beta 3)/Alginate, encapsulate hPDMCs to inject a scaffold. The chondrogenic differentiations of the construction are best than others. After 7 days the cell proliferation of the construction were maximum and higher than others, furthermore, it can maintain the maximum cell numbers until 21 days. On the other hand, the component of extracellular matrix (ECM) of the construction was maximum after 7 days, also it can maintain the maximum cell numbers until 21 days. Histological examination revealed the presence of lacuna formation in the ECM. In the same system, the chondrogenic differentiations of hPDMCs are better than hBMSCs. The results of this research suggested hPDMCs can be one of the cell sources for tissue engineering of cartilage. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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