Elevations of immunosuppressive Treg and Th17 associated with advanced disease in HNSSC
| Autor: | Meng Hua Le, 李孟樺 |
|---|---|
| Rok vydání: | 2009 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 97 The role of anti-tumor immunity in relation to head and neck squamous cell carcinoma (HNSCC) has been intensely studied in the past few years. It is now becoming clear that the complex interaction between HNSCC and immune cells plays an important part in determining tumor growth and progression. It has also led to the identification of a distinct suppressor cell population known as regulatory T cells (Treg) that play a crucial role in maintaining T-cell tolerance to self-antigens. Therefore, the current study aimed to utilize multicolor flow cytometric analysis to study the frequency and phenotype of Treg cells in peripheral blood lymphocytes (PBL) of HNSSC patients. We found that compared with healthy volunteers, the percentages of circulating CD4+CD25+ cells were increased in HNSCC patients, and this cell subset contained CD4+CD25high and CD4+CD25+Foxp3+ cells. In addition, the frequency of CD4+ cells (Th17) was also investigated, and patients with HNSCC had a higher proportion of IL17+ T cells in peripheral blood. Notably, the increased prevalence of IL17+ T cells was associated with clinical stage. Furthermore, the concentrations of IL-17、TGF-β and IL-21 cytokines in patients’ PBMC culture supernatant were significantly increased, and evaluated the potential association of Th17 cells with HNSCC. These results indicate that Treg and IL-17+ cells may contribute to tumor pathogenesis of HNSCC. Further studies of molecular mechanisms that are involved in the function of Treg and Th17 with different immune cell subsets are urgently required to help develop immunotherapeutic strategies that might overcome tumor immunsuppression. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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