Application of Surface Plasmon Resonance sensing for protein immunoassay in a micro/nano-fluidic chip
| Autor: | Lian-Jing Liou, 留連晉 |
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| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 Surface plasmon resonance (SPR) is a kind of optical detecting method and popular using in biosensor recent years. Compared with other technologies, SPR biosensor has several advantages such as real-time monitoring, label-free detection and small sample volume .Compare with the immunoassay of biomolecule, SPR sensing could provide high specificity and sensitivity in detect analytes in complex biological media. In this paper, we focus on SPR optical system’s building and application of biosensing. We discuss SPR from theory to simulation and build a SPR biosensing system by using prism coupler mode to excite Surface plasmon wave. Then we use different concentration of sucrose solution to test our SPR system and provide a good system sensitivity(147.34◦/RIU) and resolution(each CCD pixel : 3.5×10-5RIU). At last, we combine SPR system with micro/nano-fluidic chip and use the advantage of the micro channel size to enhance the detection range of biomolecule immunoassay. From the result of protein immunoassay experiment, we find out the SPR shift angle is 0.1° in bulk gold chip and it raise increase to 1.1° in micro/nano- fluidic chip. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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